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MICROBIOLOGY Unknown Number 109 and Alternative #4 Rebecca Blackmon April 30th, 2013 General Microbiology I: BIO 203 604 Spring 2013 INTRODUCTION: The way bacteria function and flourish is important, especially for people entering the Healthcare field, because it allows people to have a better understanding on how to work with or fight the bacteria. The General Microbiology I course taught students about many kinds of bacteria.
Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube. MATERIALS AND METHODS: The professor assigned the Unknown 109 test tube. Also, an Alternative #4 tube was given because the Unknown 109 tube was not allowing the gram negative to be shown on the nutrient agar.
The procedures from the laboratory manual by McDonald were used to accomplish the tests needed to find the two unknown bacteria (3). First, the bacteria mix inside the Unknown 109 tube was streaked across a nutrient agar plate using the quadrant streak technique. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye.